Ghrelin antibody (AA 47-96)

Catalog No. ABIN1453350

The Rabbit Polyclonal anti-Ghrelin antibody (ABIN1453350) specifically detects Ghrelin in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Ghrelin antibody (AA 47-96) (ABIN1453350)

Target: Ghrelin (GHRL)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ghrelin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Ghrelin Antibody

Binding Specificity: AA 47-96

Purpose: Ghrelin Antibody

Specificity: Ghrelin Polyclonal Antibody detects endogenous levels of Ghrelin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from human Ghrelin. AA range:47-96

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Ghrelin

Target: Ghrelin (GHRL)

Alternative Name: Ghrelin

Background:

Synonyms: GHRL, MTLRP, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46

Background: This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function

Gene Name: GHRL

Protein Name: Appetite-regulating hormone

Molecular Weight: 12 kDa

Gene ID: 51738

OMIM: 605353

UniProt: Q9UBU3

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Negative Regulation of Hormone Secretion, Synaptic Membrane, Feeding Behaviour