CKS1 antibody (C-Term)

Catalog No. ABIN1493317

This Rabbit Polyclonal antibody specifically detects CKS1 in WB, ELISA, IF, IHC, IHC (p) and IP. It exhibits reactivity toward Human.

Quick Overview for CKS1 antibody (C-Term) (ABIN1493317)

Target: CKS1 (CKS1B) (CDC28 Protein Kinase Regulatory Subunit 1B (CKS1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-CKS1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Reacts with the human Cks1 protein. On Western blots, it identifies a single band at ~11kD. Bands of unknown origin at ~20, 30 and 100kD are also observed. Reactivity has been confirmed with HeLa cell lysates by Western blotting, immunofluorescence and immunoprecipitation, and with non-small cell lung carcinoma tissue by immunohistochemistry. Western blotting with purified Cks1 and Cks2 proteins was used to confirm that this antibody does not cross-react with Cks2. Cross-reactivity with mouse is expected based upon amino acid sequence homology.

Cross-Reactivity (Details): Cross-reactivity with mouse is expected based upon amino acid sequence homology.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of human and mouse Cks1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (10 μg/mL), IHC, IHC-P (5 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL. Immunofluorescence: 10 μg/mL. Immunoprecipitation: 7 μg/IP reaction. Immunohistochemistry: 2.5 μg/mL. Note: For immunohistochemistry with formalin-fixed, paraffin-embedded tissues, Heat Induced Epitope Retrieval (HIER) with citrate buffer,  pH 6.0, is recommended.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CKS1

Target: CKS1 (CKS1B) (CDC28 Protein Kinase Regulatory Subunit 1B (CKS1B))

Alternative Name: CKS1B / CKS1

Background: Name/Gene ID: CKS1B
Subfamily: CKS

Synonyms: CKS1B, CDC28 protein kinase 1, CKS-1, CDC28 protein kinase 1B, Ckshs1, PNAS-18, PNAS-143, PNAS-16, CDC2-associated protein CKS1, CKS1

Gene ID: 1163

UniProt: P61024

Pathways: Mitotic G1-G1/S Phases, Inositol Metabolic Process