EPH Receptor A3 antibody (AA 38-375)

Catalog No. ABIN1498036

This Rabbit Polyclonal antibody specifically detects EPH Receptor A3 in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for EPH Receptor A3 antibody (AA 38-375) (ABIN1498036)

Target: EPH Receptor A3 (EPHA3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EPH Receptor A3 Antibody

Binding Specificity: AA 38-375

Specificity: Human EPHA3

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Porcine, Bovine (97%) Rat (96%) Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 38 and 375 of Eph Receptor A3 (SwissProt P29320). Percent identity by BLAST analysis: Human (100%), Mouse, Porcine, Bovine (97%), Rat (96%), Chicken (92%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for EPH Receptor A3

Target: EPH Receptor A3 (EPHA3)

Alternative Name: EPHA3 / EPH Receptor A3

Background: Name/Gene ID: EPHA3
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHA3, CEK4 kinase, EPH receptor A3, Ephrin type-A receptor 3, Eph-like tyrosine kinase 1, ETK1, HEK4, EPH-like kinase 4, HEK, Human embryo kinase 1, MEK4 kinase, Human embryo kinase, Tyrosine-protein kinase TYRO4, TYRO4, EK4, Eph kinase A3, ETK

Gene ID: 2042

UniProt: P29320

Pathways: RTK Signaling, Regulation of Cell Size