POLR3E antibody (AA 89-323)

Catalog No. ABIN1500341

The Rabbit Polyclonal anti-POLR3E antibody is suitable to detect POLR3E in samples from Human. It has been validated for WB, IF and ICC.

Quick Overview for POLR3E antibody (AA 89-323) (ABIN1500341)

Target: POLR3E (Polymerase (RNA) III (DNA Directed) Polypeptide E (80kD) (POLR3E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3E antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POLR3E Antibody

Binding Specificity: AA 89-323

Specificity: Human POLR3E

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat (96%) Zebrafish (82%).

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 89 and 323 of POLR3E (SwissProt Q9NVU0). Percent identity by BLAST analysis: Human (100%), Mouse, Rat (96%), Zebrafish (82%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100 - 1:1000), IF (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7, 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for POLR3E

Target: POLR3E (Polymerase (RNA) III (DNA Directed) Polypeptide E (80kD) (POLR3E))

Alternative Name: POLR3E / SIN

Background: Name/Gene ID: POLR3E

Synonyms: POLR3E, KIAA1452, RNA polymerase III subunit C5, RPC5, SIN

Gene ID: 55718

UniProt: Q9NVU0