POLR3E antibody (AA 191-240)

Catalog No. ABIN1534848

This Rabbit Polyclonal antibody specifically detects POLR3E in ELISA, IHC, WB and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for POLR3E antibody (AA 191-240) (ABIN1534848)

Target: POLR3E (Polymerase (RNA) III (DNA Directed) Polypeptide E (80kD) (POLR3E))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3E antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-POLR3E Antibody

Binding Specificity: AA 191-240

Purpose: POLR3E Antibody

Specificity: POLR3E Polyclonal Antibody detects endogenous levels of POLR3E protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human RPC5. AA range:191-240

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for POLR3E

Target: POLR3E (Polymerase (RNA) III (DNA Directed) Polypeptide E (80kD) (POLR3E))

Alternative Name: RPC5

Background:

Synonyms: POLR3E, KIAA1452, DNA-directed RNA polymerase III subunit RPC5, RNA polymerase III subunit C5, DNA-directed RNA polymerase III 80 kDa polypeptide

Background: function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters.,subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with POLR3D/RPC4.,

Gene Name: POLR3E

Protein Name: DNA-directed RNA polymerase III subunit RPC5

Molecular Weight: 79 kDa

Gene ID: 55718

UniProt: Q9NVU0