PPM1J antibody (AA 133-442)

Catalog No. ABIN1500379

This Rabbit Polyclonal antibody specifically detects PPM1J in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PPM1J antibody (AA 133-442) (ABIN1500379)

Target: PPM1J (Protein Phosphatase 1J (PPM1J))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPM1J antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PPM1J Antibody

Binding Specificity: AA 133-442

Specificity: Human PPM1J

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rat (92%) Mouse (90%).

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 133 and 442 of PPM1J (SwissProt Q5JR12). Percent identity by BLAST analysis: Human (100%), Rat (92%), Mouse (90%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7, 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for PPM1J

Target: PPM1J (Protein Phosphatase 1J (PPM1J))

Alternative Name: PPM1J

Background: Name/Gene ID: PPM1J
Family: Ras GTPase superfamily IPR001806

Synonyms: PPM1J, PP2C-zeta, PP2CZ, PPP2CZ, Protein phosphatase 2C zeta, RP11-426L16.5, Transforming protein rhoc, Protein phosphatase 1J, PP2Czeta

Gene ID: 333926

UniProt: Q5JR12