PKC theta antibody (AA 15-294)

Catalog No. ABIN1501904

The Rabbit Polyclonal anti-PKC theta antibody has been validated for WB, IHC and IHC (p). It is suitable to detect PKC theta in samples from Human.

Quick Overview for PKC theta antibody (AA 15-294) (ABIN1501904)

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC theta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PKC theta Antibody

Binding Specificity: AA 15-294

Specificity: Human, mouse PRKCQ / PKC-Theta

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine (98%).

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 15 and 294 of PKC theta (SwissProt Q04759). Percent identity by BLAST analysis: Human (100%), Bovine (98%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7, 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for PKC theta

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Alternative Name: PRKCQ / PKC-Theta

Background: Name/Gene ID: PRKCQ
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCQ, NPKC-theta, PKC theta, PRKCT, Protein kinase C theta type, Protein kinase c theta, PKCtheta, Protein kinase C, theta

Gene ID: 5588

UniProt: Q04759

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling