GAPDH antibody

Catalog No. ABIN1512934

This Mouse Monoclonal antibody specifically detects GAPDH in WB, IHC and IF. It exhibits reactivity toward Human and has been mentioned in 46+ publications.

Quick Overview for GAPDH antibody (ABIN1512934)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GAPDH Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Loading control Antibodies

Purification: Affinity purification

Immunogen: Recombinant protein of human GAPDH

Isotype: IgG

Application Details

Application Notes: WB,1:5000 - 1:20000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-GAPDH antibody (ABIN1512934)

Wu, Xiong, Li, Yang, Ge et al.: "Integrated Proteomic and Transcriptomic Analysis Reveals Long Noncoding RNA HOX Transcript Antisense Intergenic RNA (HOTAIR) Promotes Hepatocellular Carcinoma Cell Proliferation by Regulating Opioid ..." in: Molecular & cellular proteomics : MCP, Vol. 17, Issue 1, pp. 146-159, (2019) (PubMed).

Han, Cai, Gao, Meng, Gao, Wu, Duan, Wang, Dinislam, Lin, Kang, Jiang: "Loss of ATRX suppresses ATM dependent DNA damage repair by modulating H3K9me3 to enhance temozolomide sensitivity in glioma." in: Cancer letters, Vol. 419, pp. 280-290, (2019) (PubMed).

Zhang, Qian, Zheng, Qian, Wang, Thu, Zhang, Zhou, Tu, Liu, Li, Yang, Li, Xu: "Jujuboside A promotes Aβ clearance and ameliorates cognitive deficiency in Alzheimer's disease through activating Axl/HSP90/PPARγ pathway." in: Theranostics, Vol. 8, Issue 15, pp. 4262-4278, (2018) (PubMed).

Yu, Chen, Li, Yang, Zhang, Huangfu, Zheng, Zhao, Lv, Hong, Liang, Shan: "YAP1 contributes to NSCLC invasion and migration by promoting Slug transcription via the transcription co-factor TEAD." in: Cell death & disease, Vol. 9, Issue 5, pp. 464, (2018) (PubMed).

Peng, Wang, Shu, Yang, Wang, Ouyang, Shen, Hou, Zhao, Wang, Zhang, Liu, Zhao: "Raddeanin a Suppresses Glioblastoma Growth by Inducing ROS Generation and Subsequent JNK Activation to Promote Cell Apoptosis." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 47, Issue 3, pp. 1108-1121, (2018) (PubMed).

Nie, Huang, Deng, Bao, Liu, Feng, Wang, Chen, Qi, Pan: "The purine receptor P2X7R regulates the release of pro-inflammatory cytokines in human craniopharyngioma." in: Endocrine-related cancer, Vol. 24, Issue 6, pp. 287-296, (2018) (PubMed).

Wan, Ashraf, Ye, Duan, Zohaib, Wang, Chen, Zhu, Li, Chen, Cao: "MicroRNA-22 negatively regulates poly(I:C)-triggered type I interferon and inflammatory cytokine production via targeting mitochondrial antiviral signaling protein (MAVS)." in: Oncotarget, Vol. 7, Issue 47, pp. 76667-76683, (2018) (PubMed).

Wu, Lou, Ge, Xiong: "Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos." in: Scientific reports, Vol. 7, Issue 1, pp. 4022, (2018) (PubMed).

Lei, Pan, Li, Zhou, Zhang, He, Peng, Li, Sidhu, Chen, Hua: "H19 regulates the proliferation of bovine male germline stem cells via IGF-1 signaling pathway." in: Journal of cellular physiology, Vol. 234, Issue 1, pp. 915-926, (2018) (PubMed).

Dong, Xu, Yang, Shi, Jiang, Chen, Song, Wang: "Associations of Functional MicroRNA Binding Site Polymorphisms in IL23/Th17 Inflammatory Pathway Genes with Gastric Cancer Risk." in: Mediators of inflammation, Vol. 2017, pp. 6974696, (2018) (PubMed).

Li, Zhu, Yang, Dib-Hajj, Waxman, Yu, Xu, Cheng: "Nav1.7 is phosphorylated by Fyn tyrosine kinase which modulates channel expression and gating in a cell type-dependent manner." in: Molecular pain, Vol. 14, pp. 1744806918782229, (2018) (PubMed).

Zhang, Zhou, Huang, Yu, Zhang, Wang: "Placental mechanism of prenatal nicotine exposure-reduced blood cholesterol levels in female fetal rats." in: Toxicology letters, Vol. 296, pp. 31-38, (2018) (PubMed).

Sheng, Yu, Chen, Wang, Lu: "Infection of grass carp reovirus induced the expressional suppression of pro-viral Fibulin-4 in host cells." in: Fish & shellfish immunology, Vol. 77, pp. 294-297, (2018) (PubMed).

Zhang, Zhang, Deng, Zhang, Liu, Xin, Li, Xu: "Baicalin attenuates non-alcoholic steatohepatitis by suppressing key regulators of lipid metabolism, inflammation and fibrosis in mice." in: Life sciences, Vol. 192, pp. 46-54, (2018) (PubMed).

Liu, Song, Zhao, He, Wang, Chen, Xiang, Yi, Qi, Liu: "Antitumor Activity and Mechanism of a Reverse Transcriptase Inhibitor, Dapivirine, in Glioblastoma." in: Journal of Cancer, Vol. 9, Issue 1, pp. 117-128, (2018) (PubMed).

Liu, Zhang, Yao, Xie, Dai, Zhang, Zhou: "Serum extracellular vesicles promote proliferation of H9C2 cardiomyocytes by increasing miR-17-3p." in: Biochemical and biophysical research communications, Vol. 499, Issue 3, pp. 441-446, (2018) (PubMed).

Li, Zhou, Yang, Li, Zhang, Wang, Jiao: "SGK1 inhibits PM2.5-induced apoptosis and oxidative stress in human lung alveolar epithelial A549 cells." in: Biochemical and biophysical research communications, Vol. 496, Issue 4, pp. 1291-1295, (2018) (PubMed).

Ruan, Zhao, Zhao, Zhang, Shi, Pang: "Knockdown of long noncoding RNA MEG3 impairs VEGF-stimulated endothelial sprouting angiogenesis via modulating VEGFR2 expression in human umbilical vein endothelial cells." in: Gene, Vol. 649, pp. 32-39, (2018) (PubMed).

Qiu, Wu, Meng, Luo, Zhao, Sun, Gao: "GYY4137 protects against myocardial ischemia/reperfusion injury via activation of the PHLPP-1/Akt/Nrf2 signaling pathway in diabetic mice." in: The Journal of surgical research, Vol. 225, pp. 29-39, (2018) (PubMed).

Shen, Zhu, Kang, Tu, Li, Zhang, Qin, Yang, Guan: "Lanosterol Synthase Pathway Alleviates Lens Opacity in Age-Related Cortical Cataract." in: Journal of ophthalmology, Vol. 2018, pp. 4125893, (2018) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.,G3PD,GAPD,HEL-S-162eP,GAPDH,Cancer,Signal Transduction,Endocrine & Metabolism,Carbohydrate metabolism,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease,GAPDH

Molecular Weight: 31 kDa/36 kDa

Gene ID: 2597

UniProt: P04406