alpha Adducin antibody (pSer726)

Catalog No. ABIN1531171

The Rabbit Polyclonal anti-alpha Adducin antibody is suitable to detect alpha Adducin in samples from Human, Mouse and Rat. It has been validated for IHC, WB, ELISA and IF.

Quick Overview for alpha Adducin antibody (pSer726) (ABIN1531171)

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Adducin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-alpha Adducin Antibody

Binding Specificity: AA 688-737, pSer726

Purpose: Adducin α/β (phospho Ser726/713) Antibody

Specificity: Phospho-Adducin α/β (S726/713) Polyclonal Antibody detects endogenous levels of Adducin α/β protein only when phosphorylated at S726/713.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ADD1 around the phosphorylation site of Ser726. AA range:688-737

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for alpha Adducin

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Alternative Name: ADD1

Background:

Synonyms: ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha, ADD2, ADDB, Beta-adducin, Erythrocyte adducin subunit beta

Background: adducin 1(ADD1) Homo sapiens Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described. [provided by RefSeq, Jul 2008],

Gene Name: ADD1/ADD2

Protein Name: Alpha-adducin/Beta-adducin

Molecular Weight: 80 kDa

Gene ID: 118, 119

OMIM: 102680

UniProt: P35611

Pathways: Negative Regulation of Hormone Secretion, Regulation of Actin Filament Polymerization, Regulation of Lipid Metabolism by PPARalpha, ER-Nucleus Signaling