IkappaB-epsilon antibody (pSer22)
This anti-IkappaB-epsilon antibody is a Rabbit Polyclonal antibody detecting IkappaB-epsilon in IF, IHC, ELISA and WB. Suitable for Human, Mouse and Rat.
Quick Overview for IkappaB-epsilon antibody (pSer22) (ABIN1531184)
Target
IkappaB-epsilon
Reactivity
Human, Mouse, Rat
Host
Rabbit
Clonality
Polyclonal
Conjugate
This IkappaB-epsilon antibody is un-conjugated
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Binding Specificity
- AA 131-180, pSer22
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Specificity
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IkappaB-epsilon (Phospho-Ser22) Antibody detects endogenous levels of IkappaB-epsilon only when phosphorylated at Ser22.
PhosphorylationH:S22 M:S22 R:S22
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Purification
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Purity
- > 95 %
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Immunogen
- The antiserum was produced against synthesized peptide derived from human IkappaB-epsilon around the phosphorylation site of Ser22.
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Isotype
- IgG
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Application Notes
- WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
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Comment
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Unigene-Number: Hs.458276 (NCBI Gene Symbol: NFKBIE)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Stable at -20°C for at least 1 year.
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Expiry Date
- 12 months
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- IkappaB-epsilon
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Background
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Synonyms: I-kappa-B-epsilon, IKBE, IkappaBepsilon, NF-kappa-BIE, NF-kappaB inhibitor epsilon, NFKBIE
NCBI Gene Symbol: NFKBIE
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Molecular Weight
- 38 kDa
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Gene ID
- 4794
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OMIM
- 604548
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UniProt
- O00221
Target
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