IkappaB-epsilon antibody (pSer22)

Catalog No. ABIN7222756

This Rabbit Polyclonal antibody specifically detects IkappaB-epsilon in IF, IHC, ELISA and WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IkappaB-epsilon antibody (pSer22) (ABIN7222756)

Target: IkappaB-epsilon

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IkappaB-epsilon antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Product Details anti-IkappaB-epsilon Antibody

Binding Specificity: pSer22

Purpose: Rabbit Anti-IκB-ε (phospho Ser22) Polyclonal Antibody

Specificity: Phospho-IκB-ε (S22) Polyclonal Antibody detects endogenous levels of IκB-ε protein only when phosphorylated at S22.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human IkappaB-epsilon Phospho-Ser22

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IkappaB-epsilon

Target: IkappaB-epsilon

Background: NFKBIE, IKBE, NF-kappa-B inhibitor epsilon, NF-kappa-BIE, I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilonThe protein encoded by NFKBIE binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus.

Molecular Weight: 38kD

Gene ID: 4794

UniProt: O00221