Cytokeratin 18 antibody (pSer34)

Catalog No. ABIN1531185

This Rabbit Polyclonal antibody specifically detects Cytokeratin 18 in WB, IHC, IF and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cytokeratin 18 antibody (pSer34) (ABIN1531185)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-Cytokeratin 18 Antibody

Binding Specificity: AA 1-50, pSer34

Specificity:

Keratin 18 (Phospho-Ser33) Antibody detects endogenous levels of Keratin 18 only when phosphorylated at Ser33.

PhosphorylationH:S34 M:S35 R:S35

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Keratin 18 around the phosphorylation site of Ser33.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

Comment:

Unigene-Number: Hs.406013 (NCBI Gene Symbol: KRT18)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Keratin 18

Background:

Synonyms: CK 18, CK18, CYK18, Cytokeratin endo B, K18, K1C18, KERD, KRT1-18, KRT18, Keratin, Keratin D, Keratin, type I cytoskeletal 18, cytokeratin 18, type I cytoskeletal 18

NCBI Gene Symbol: KRT18

Molecular Weight: 47 kDa

Gene ID: 3875

OMIM: 148070

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis