PKC zeta antibody (pThr410)

Catalog No. ABIN1531192

The Rabbit Polyclonal anti-PKC zeta antibody is suitable to detect PKC zeta in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for PKC zeta antibody (pThr410) (ABIN1531192)

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC zeta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PKC zeta Antibody

Binding Specificity: AA 376-425, pThr410

Specificity:

PKC zeta (Phospho-Thr410) Antibody detects endogenous levels of PKC zeta only when phosphorylated at Thr410.

PhosphorylationH:T410 M:T410 R:T410

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC zeta around the phosphorylation site of Thr410.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

Comment:

Unigene-Number: Hs.496255 (NCBI Gene Symbol: PRKCZ)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PKC zeta

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Alternative Name: PKC zeta

Background:

Synonyms: KPCZ, PKC-zeta, PKC2, PRKCZ, Protein kinase C zeta type, kinase PKC-zeta, nPKC-zeta

NCBI Gene Symbol: PRKCZ

Molecular Weight: 67 kDa

Gene ID: 5590

OMIM: 176982

UniProt: Q05513

Pathways: NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling