Acetyl-CoA Carboxylase antibody (pSer80)

Catalog No. ABIN1531208

The Rabbit Polyclonal anti-Acetyl-CoA Carboxylase antibody (ABIN1531208) specifically detects Acetyl-CoA Carboxylase in WB, IHC, ELISA and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Acetyl-CoA Carboxylase antibody (pSer80) (ABIN1531208)

Target: Acetyl-CoA Carboxylase

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetyl-CoA Carboxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Acetyl-CoA Carboxylase Antibody

Binding Specificity: AA 46-95, pSer80

Purpose: ACCα (phospho Ser80) Antibody

Specificity: Phospho-ACCα (S80) Polyclonal Antibody detects endogenous levels of ACCα protein only when phosphorylated at S80.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Acetyl-CoA Carboxylase around the phosphorylation site of Ser80. AA range:46-95

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Acetyl-CoA Carboxylase

Target: Acetyl-CoA Carboxylase

Background:

Synonyms: ACACA; ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha

Background: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Gene Name: ACACA

Protein Name: Acetyl-CoA carboxylase 1

Molecular Weight: 265 kDa

Gene ID: 31

OMIM: 200350

UniProt: Q13085