Phospholipase C gamma 2 antibody (pTyr753)

Catalog No. ABIN1531237

The Rabbit Polyclonal anti-Phospholipase C gamma 2 antibody has been validated for WB, IHC and ELISA. It is suitable to detect Phospholipase C gamma 2 in samples from Human, Rat and Mouse.

Quick Overview for Phospholipase C gamma 2 antibody (pTyr753) (ABIN1531237)

Target: Phospholipase C gamma 2 (PLCG2)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C gamma 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Phospholipase C gamma 2 Antibody

Binding Specificity: AA 721-770, pTyr753

Specificity:

PLCG2 (Phospho-Tyr753) Antibody detects endogenous levels of PLCG2 only when phosphorylated at Tyr753.

PhosphorylationH:Y753 M:Y753 R:Y753

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PLCG2 around the phosphorylation site of Tyr753.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

Comment:

Unigene-Number: Hs.413111 (NCBI Gene Symbol: PLCG2)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Phospholipase C gamma 2

Target: Phospholipase C gamma 2 (PLCG2)

Alternative Name: PLCG2

Background:

Synonyms: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 2, phospholipase C-gamma-2, PLC-gamma2, PLC-IV

NCBI Gene Symbol: PLCG2

Molecular Weight: 147 kDa

Gene ID: 5336

OMIM: 600220

UniProt: P16885

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling