POLR2A/RPB1 antibody (pSer1619)

Catalog No. ABIN1531238

Product Details anti-POLR2A/RPB1 Antibody

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Binding Specificity: AA 1585-1634, pSer1619

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Specificity:

POLR2A (Phospho-Ser1619) Antibody detects endogenous levels of POLR2A only when phosphorylated at Ser1619.

PhosphorylationH:S1619 M:S1619 R:S1619

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human POLR2A around the phosphorylation site of Ser1619.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

Comment:

Unigene-Number: Hs.270017 (NCBI Gene Symbol: RPB1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A (POLR2A Products)

Background:

Synonyms: DNA-directed RNA polymerase II largest subunit, RNA pol II largest subunit, RNA polymerase II 220 kd subunit, RPB1

NCBI Gene Symbol: RPB1

Molecular Weight: 217 kDa

Gene ID: 5430

OMIM: 180660

UniProt: P24928

Pathways: Regulatory RNA Pathways