PTK2B antibody (pTyr580)

Catalog No. ABIN1531240

The Rabbit Polyclonal anti-PTK2B antibody has been validated for WB, IHC and ELISA. It is suitable to detect PTK2B in samples from Human, Mouse and Rat.

Quick Overview for PTK2B antibody (pTyr580) (ABIN1531240)

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTK2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-PTK2B Antibody

Binding Specificity: AA 546-595, pTyr580

Specificity:

PYK2 (Phospho-Tyr580) Antibody detects endogenous levels of PYK2 only when phosphorylated at Tyr580.

PhosphorylationH:Y580 M:Y580 R:Y580

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PYK2 around the phosphorylation site of Tyr580.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

Comment:

Unigene-Number: Hs.491322 (NCBI Gene Symbol: PTK2B)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PTK2B

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Alternative Name: PYK2

Background:

Synonyms: CADTK, CAK beta, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, FADK 2, FAK2, Focal adhesion kinase 2, kinase Pyk2, Proline-rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK2B, RAFTK, Related adhesion focal tyrosine kinase

NCBI Gene Symbol: PTK2B

Molecular Weight: 115 kDa

Gene ID: 2185

OMIM: 601212

UniProt: Q14289

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling