SRF antibody (pSer99)

Catalog No. ABIN1531244

The Rabbit Polyclonal anti-SRF antibody (ABIN1531244) specifically detects SRF in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SRF antibody (pSer99) (ABIN1531244)

Target: SRF (Serum Response Factor (SRF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-SRF Antibody

Binding Specificity: AA 71-120, pSer99

Purpose: SRF (phospho Ser103) Antibody

Specificity: Phospho-SRF (S103) Polyclonal Antibody detects endogenous levels of SRF protein only when phosphorylated at S103.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human SRF around the phosphorylation site of Ser99. AA range:71-120

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for SRF

Target: SRF (Serum Response Factor (SRF))

Alternative Name: SRF

Background:

Synonyms: SRF, Serum response factor, SRF

Background: This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014],

Gene Name: SRF

Protein Name: Serum response factor

Molecular Weight: 51 kDa

Gene ID: 6722

OMIM: 600589

UniProt: P11831