HDAC5 antibody (pSer259)

Catalog No. ABIN1531281

This Rabbit Polyclonal antibody specifically detects HDAC5 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for HDAC5 antibody (pSer259) (ABIN1531281)

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-HDAC5 Antibody

Binding Specificity: AA 225-274, pSer259

Purpose: HDAC5/9 (phospho Ser259/220) Antibody

Specificity: Phospho-HDAC5/9 (S259/220) Polyclonal Antibody detects endogenous levels of HDAC5/9 protein only when phosphorylated at S259/220.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HDAC5 around the phosphorylation site of Ser259. AA range:225-274

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for HDAC5

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Alternative Name: HDAC5

Background:

Synonyms: HDAC5, KIAA0600, Histone deacetylase 5, HD5, Antigen NY-CO-9, HDAC9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR, Histone deacetylase 9, HD9, Histone deacetylase 7B, HD7, HD7b, Histone deacetylase-related protein, MEF2-interacting transcription rep

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA.The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Gene Name: HDAC5/HDAC9

Protein Name: Histone deacetylase 5/9

Molecular Weight: 121 kDa

Gene ID: 10014

OMIM: 605315

UniProt: Q9UQL6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process