IKK-alpha /IKK-beta antibody (pSer180, pSer181)

Catalog No. ABIN1531284

This Rabbit Polyclonal antibody specifically detects IKK-alpha /IKK-beta in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for IKK-alpha /IKK-beta antibody (pSer180, pSer181) (ABIN1531284)

Target: IKK-alpha /IKK-beta

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK-alpha /IKK-beta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-IKK-alpha /IKK-beta Antibody

Binding Specificity: AA 151-200, pSer180, pSer181

Purpose: IKKα/β (phospho Ser180/181) Antibody

Specificity: Phospho-IKKα/β (S180/181) Polyclonal Antibody detects endogenous levels of IKKα/β protein only when phosphorylated at S180/181.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta around the phosphorylation site of Ser180/181. AA range:151-200

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

References for anti-IKK-alpha /IKK-beta antibody (ABIN1531284)

Hsieh, Hsiao, Hsu, Wang, Sheu: "PMC, a potent hydrophilic ?-tocopherol derivative, inhibits NF-?B activation via PP2A but not I?B?-dependent signals in vascular smooth muscle cells." in: Journal of cellular and molecular medicine, Vol. 18, Issue 7, pp. 1278-89, (2014) (PubMed).

Target Details for IKK-alpha /IKK-beta

Target: IKK-alpha /IKK-beta

Alternative Name: IKK-alpha/beta

Background:

Synonyms: CHUK, IKKA, TCF16, Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, IKK1, Nuclear factor NF-kappa-B

Background: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],

Gene Name: CHUK/IKBKB

Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit alpha

Molecular Weight: 84 kDa

Gene ID: 1147, 3551

OMIM: 600664

UniProt: O15111