CDK1 antibody (pThr14)

Catalog No. ABIN1531310

The Rabbit Polyclonal anti-CDK1 antibody (ABIN1531310) specifically detects CDK1 in WB, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CDK1 antibody (pThr14) (ABIN1531310)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-CDK1 Antibody

Binding Specificity: AA 1-50, pThr14

Specificity:

CDK1/CDC2 (Phospho-Thr14) Antibody detects endogenous levels of CDK1/CDC2 only when phosphorylated at Thr14.

PhosphorylationH:T14 M:T14 R:T14

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CDK1/CDC2 around the phosphorylation site of Thr14.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

Comment:

Unigene-Number: Hs.19192, Hs.689624 (NCBI Gene Symbol: CDK2)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDK1/CDC2

Background:

Synonyms: Cell division protein kinase 2, kinase Cdk2, p33 protein kinase

NCBI Gene Symbol: CDK2

Molecular Weight: 33 kDa

Gene ID: 1017

OMIM: 116953

UniProt: P24941

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA