JNK antibody (pThr183)

Catalog No. ABIN481942

This Rabbit Polyclonal antibody specifically detects JNK in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for JNK antibody (pThr183) (ABIN481942)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-JNK Antibody

Binding Specificity: AA 151-200, pThr183

Specificity:

JNK1/2/3 (Phospho-Thr183+Tyr185) Antibody detects endogenous levels of JNK1/2/3 only when phosphorylated at Thr183 and Tyr185.

PhosphorylationH:T183/Y185 M:T183/Y185 R:T183/Y185

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human JNK1/2/3 around the phosphorylation site of Thr183 and Tyr185.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:10000

Comment:

Unigene-Number: Hs.138211, Hs.654461, Hs.125503 (NCBI Gene Symbol: MAPK8)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: JNK1/2/3

Background:

Synonyms: JNK-46, JNK1-alpha-2, MAPK8, MK08, Mitogen-activated protein kinase 8, PRKM8, SAPK gamma, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, kinase JNK1, p54 gamma

NCBI Gene Symbol: MAPK8

Molecular Weight: 48 kDa

Gene ID: 5599, 5601, 5602

OMIM: 601158

UniProt: P45983, P45984, P53779

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins