NOS1 antibody (pSer852)

Catalog No. ABIN1531354

Product Details anti-NOS1 Antibody

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Binding Specificity: AA 818-867, pSer852

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Specificity:

n-NOS (Phospho-Ser852) Antibody detects endogenous levels of n-NOS only when phosphorylated at Ser852.

PhosphorylationH:S852 M:S847 R:S847

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human n-NOS around the phosphorylation site of Ser852.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

Comment:

Unigene-Number: Hs.654410 (NCBI Gene Symbol: NOS1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: N-NOS (NOS1 Products)

Background:

Synonyms: bNOS, BNOS, Constitutive NOS, N-NOS, NC-NOS, Neuronal NOS, Nitric-oxide synthase, brain, NOS, type I, NOS1

NCBI Gene Symbol: NOS1

Molecular Weight: 160 kDa

Gene ID: 4842

OMIM: 163731

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction