NOS1 antibody (pSer852)

Catalog No. ABIN1531354

The Rabbit Polyclonal anti-NOS1 antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect NOS1 in samples from Human, Rat and Mouse.

Quick Overview for NOS1 antibody (pSer852) (ABIN1531354)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-NOS1 Antibody

Binding Specificity: AA 818-867, pSer852

Specificity:

n-NOS (Phospho-Ser852) Antibody detects endogenous levels of n-NOS only when phosphorylated at Ser852.

PhosphorylationH:S852 M:S847 R:S847

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human n-NOS around the phosphorylation site of Ser852.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

Comment:

Unigene-Number: Hs.654410 (NCBI Gene Symbol: NOS1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: N-NOS

Background:

Synonyms: bNOS, BNOS, Constitutive NOS, N-NOS, NC-NOS, Neuronal NOS, Nitric-oxide synthase, brain, NOS, type I, NOS1

NCBI Gene Symbol: NOS1

Molecular Weight: 160 kDa

Gene ID: 4842

OMIM: 163731

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction