GTF2I antibody (pTyr248)

Catalog No. ABIN1531401

This Rabbit Polyclonal antibody specifically detects GTF2I in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GTF2I antibody (pTyr248) (ABIN1531401)

Target: GTF2I (General Transcription Factor III (GTF2I))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2I antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GTF2I Antibody

Binding Specificity: AA 214-263, pTyr248

Purpose: TFII-I (phospho Tyr248) Antibody

Specificity: Phospho-TFII-I (Y248) Polyclonal Antibody detects endogenous levels of TFII-I protein only when phosphorylated at Y248.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human TFII-I around the phosphorylation site of Tyr248. AA range:214-263

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for GTF2I

Target: GTF2I (General Transcription Factor III (GTF2I))

Alternative Name: TFII-I

Background:

Synonyms: GTF2I, BAP135, WBSCR6, General transcription factor II-I, GTFII-I, TFII-I, Bruton tyrosine kinase-associated protein 135, BAP-135, BTK-associated protein 135, SRF-Phox1-interacting protein, SPIN, Williams-Beuren syndrome chromosomal region

Background: general transcription factor IIi(GTF2I) Homo sapiens This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013],

Gene Name: GTF2I

Protein Name: General transcription factor II-I

Molecular Weight: 112 kDa

Gene ID: 2969

OMIM: 194050

UniProt: P78347