TNNI3 antibody (pSer22, pSer23)

Catalog No. ABIN1531404

This Rabbit Polyclonal antibody specifically detects TNNI3 in WB, IHC, ELISA and IF. It exhibits reactivity toward Mouse and Rat.

Quick Overview for TNNI3 antibody (pSer22, pSer23) (ABIN1531404)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-TNNI3 Antibody

Binding Specificity: AA 5-54, pSer22, pSer23

Purpose: Troponin I-C (phospho Ser22/S23) Antibody

Specificity: Phospho-Troponin I-C (S22/S23) Polyclonal Antibody detects endogenous levels of Troponin I-C protein only when phosphorylated at S22/S23.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from mouse TNNI3 around the phosphorylation site of Ser22 and Ser23. AA range:5-54

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: TNNI3

Background:

Synonyms: TNNI3, TNNC1, Troponin I, cardiac muscle, Cardiac troponin I

Background: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac.This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

Gene Name: Tnni3

Protein Name: Troponin I cardiac muscle

Molecular Weight: 24 kDa

UniProt: P19429