WASP antibody (pTyr290)

Catalog No. ABIN1531412

The Rabbit Polyclonal anti-WASP antibody (ABIN1531412) specifically detects WASP in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for WASP antibody (pTyr290) (ABIN1531412)

Target: WASP (WAS) (Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WASP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-WASP Antibody

Binding Specificity: AA 256-305, pTyr290

Purpose: WASP (phospho Tyr290) Antibody

Specificity: Phospho-WASP (Y290) Polyclonal Antibody detects endogenous levels of WASP protein only when phosphorylated at Y290.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human WASP around the phosphorylation site of Tyr290. AA range:256-305

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for WASP

Target: WASP (WAS) (Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS))

Alternative Name: WASP

Background:

Synonyms: WAS, IMD2, Wiskott-Aldrich syndrome protein, WASp

Background: The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A t

Gene Name: WAS

Protein Name: Wiskott-Aldrich syndrome protein

Molecular Weight: 52 kDa

Gene ID: 7454

OMIM: 300299

UniProt: P42768