ZAP70 antibody (pTyr315)

Catalog No. ABIN1531416

This Rabbit Polyclonal antibody specifically detects ZAP70 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for ZAP70 antibody (pTyr315) (ABIN1531416)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-ZAP70 Antibody

Binding Specificity: AA 281-330, pTyr315

Specificity:

ZAP-70 (Phospho-Tyr315) Antibody detects endogenous levels of ZAP-70 only when phosphorylated at Tyr315.

PhosphorylationH:Y315 M:Y314

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr315.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

Comment:

Unigene-Number: Hs.234569 (NCBI Gene Symbol: ZAP70)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP-70

Background:

Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70, ZAP70, kinase ZAP70

NCBI Gene Symbol: ZAP70

Molecular Weight: 69 kDa

Gene ID: 7535

OMIM: 176947

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway