ACLY antibody (pSer454)

Catalog No. ABIN1531422

The Rabbit Polyclonal anti-ACLY antibody (ABIN1531422) specifically detects ACLY in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for ACLY antibody (pSer454) (ABIN1531422)

Target: ACLY (ATP Citrate Lyase (ACLY))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACLY antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ACLY Antibody

Binding Specificity: AA 420-469, pSer454

Purpose: ATP-citrate synthase (phospho Ser455) Antibody

Specificity: Phospho-ATP-citrate synthase (S455) Polyclonal Antibody detects endogenous levels of ATP-citrate synthase protein only when phosphorylated at S455.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ATP-Citrate Lyase around the phosphorylation site of Ser454. AA range:420-469

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ACLY

Target: ACLY (ATP Citrate Lyase (ACLY))

Alternative Name: ATP-Citrate Lyase

Background:

Synonyms: ACLY, ATP-citrate synthase, ATP-citrate, pro-S-)-lyase, ACL, Citrate cleavage enzyme

Background: ATP citrate lyase(ACLY) Homo sapiens ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014],

Gene Name: ACLY

Protein Name: ATP-citrate synthase

Molecular Weight: 120 kDa

Gene ID: 47

OMIM: 108728

UniProt: P53396

Pathways: Warburg Effect