EIF4B antibody (pSer422)

Catalog No. ABIN1531431

The Rabbit Polyclonal anti-EIF4B antibody (ABIN1531431) specifically detects EIF4B in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for EIF4B antibody (pSer422) (ABIN1531431)

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-EIF4B Antibody

Binding Specificity: AA 388-437, pSer422

Purpose: eIF4B (phospho Ser422) Antibody

Specificity: Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human eIF4B around the phosphorylation site of Ser422. AA range:388-437

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Alternative Name: eIF4B

Background:

Synonyms: EIF4B, Eukaryotic translation initiation factor 4B, eIF-4B

Background: function:Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Self-associates and interacts with EIF3 p170 subunit.,

Gene Name: EIF4B

Protein Name: Eukaryotic translation initiation factor 4B

Molecular Weight: 69 kDa

Gene ID: 3716

OMIM: 147795

UniProt: P23588