BCAR1 antibody (pTyr165)

Catalog No. ABIN1531452

Product Details anti-BCAR1 Antibody

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Binding Specificity: AA 131-180, pTyr165

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity:

p130 Cas (Phospho-Tyr165) Antibody detects endogenous levels of p130 Cas only when phosphorylated at Tyr165.

PhosphorylationH:Y165 M:Y169 R:Y263

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr165.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

Comment:

Unigene-Number: Hs.479747 (NCBI Gene Symbol: BCAR1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: p130 Cas (BCAR1 Products)

Background:

Synonyms: BCA1, BCAR1, Breast cancer anti-estrogen resistance 1 protein, CAS, CRK-associated substrate, CRKAS

NCBI Gene Symbol: BCAR1

Molecular Weight: 93 kDa

Gene ID: 9564

OMIM: 602941

UniProt: P56945

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling