BCAR1 antibody (pTyr249)

Catalog No. ABIN1531453

This Rabbit Polyclonal antibody specifically detects BCAR1 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BCAR1 antibody (pTyr249) (ABIN1531453)

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-BCAR1 Antibody

Binding Specificity: AA 215-264, pTyr249

Specificity:

p130 Cas (Phospho-Tyr249) Antibody detects endogenous levels of p130 Cas only when phosphorylated at Tyr249.

PhosphorylationH:Y249 M:Y253 R:Y347

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr249.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.479747 (NCBI Gene Symbol: BCAR1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: p130 Cas

Background:

Synonyms: BCA1, BCAR1, Breast cancer anti-estrogen resistance 1 protein, CAS, CRK-associated substrate, CRKAS

NCBI Gene Symbol: BCAR1

Molecular Weight: 93 kDa

Gene ID: 9564

OMIM: 602941

UniProt: P56945

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling