PKC delta antibody (pThr507)

Catalog No. ABIN1531463

The Rabbit Polyclonal anti-PKC delta antibody has been validated for WB, IHC and ELISA. It is suitable to detect PKC delta in samples from Human, Mouse and Rat.

Quick Overview for PKC delta antibody (pThr507) (ABIN1531463)

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC delta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-PKC delta Antibody

Binding Specificity: AA 471-520, pThr507

Specificity:

PKC delta (Phospho-Thr505) Antibody detects endogenous levels of PKC delta only when phosphorylated at Thr505.

PhosphorylationH:T507 M:T505 R:T505

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC delta around the phosphorylation site of Thr505.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.155342 (NCBI Gene Symbol: PRKCD)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PKC delta

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Alternative Name: PKC delta

Background:

Synonyms: KPCD, PKC-delta, PRKCD, kinase PKC-delta, nPKC-delta

NCBI Gene Symbol: PRKCD

Molecular Weight: 77 kDa

Gene ID: 5580

OMIM: 176977

UniProt: Q05655

Pathways: Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism