PKC theta antibody (pThr538)

Catalog No. ABIN1531465

This Rabbit Polyclonal antibody specifically detects PKC theta in WB, IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for PKC theta antibody (pThr538) (ABIN1531465)

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC theta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-PKC theta Antibody

Binding Specificity: AA 504-553, pThr538

Specificity:

PKC thet (Phospho-Thr538) Antibody detects endogenous levels of PKC thet only when phosphorylated at Thr538.

PhosphorylationH:T538 M:T538 R:T538

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC thet around the phosphorylation site of Thr538.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

Comment:

Unigene-Number: Hs.498570 (NCBI Gene Symbol: PRKCQ)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PKC theta

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Alternative Name: PKC theta

Background:

Synonyms: KPCT, PKC-theta, PKCQ, PRKCQ, PRKCT, Protein kinase C, theta type, kinase PKC-theta, nPKC-theta

NCBI Gene Symbol: PRKCQ

Molecular Weight: 81 kDa

Gene ID: 5588

OMIM: 600448

UniProt: Q04759

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling