PFKFB2 antibody (pSer483)

Catalog No. ABIN1531479

The Rabbit Polyclonal anti-PFKFB2 antibody (ABIN1531479) specifically detects PFKFB2 in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PFKFB2 antibody (pSer483) (ABIN1531479)

Target: PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKFB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PFKFB2 Antibody

Binding Specificity: AA 451-500, pSer483

Specificity:

PFKFB2 (Phospho-Ser483) Antibody detects endogenous levels of PFKFB2 only when phosphorylated at Ser483.

PhosphorylationH:S483 M:S486 R:S486

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PFKFB2 around the phosphorylation site of Ser483.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

Comment:

Unigene-Number: Hs.282702 (NCBI Gene Symbol: F262)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PFKFB2

Target: PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))

Alternative Name: PFKFB2

Background:

Synonyms: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2, PFK-2/FBPase-2, 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme, 6-phosphofructo-2-kinase, Fructose-2,6-bisphosphatase, PFKFB2

NCBI Gene Symbol: F262

Molecular Weight: 58 kDa

Gene ID: 5208

OMIM: 171835

UniProt: O60825

Pathways: PI3K-Akt Signaling, Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process