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Actin antibody (pTyr55)

This Rabbit Polyclonal antibody specifically detects Actin in IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Actin-pan (alpha/gamma) (Phospho-Tyr55/53) Antibody. (Actin antibody (pTyr55))

IHC

1 image

Catalog No. ABIN1531480

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Quick Overview for Actin antibody (pTyr55) (ABIN1531480)

Target

See all Actin Antibodies

Actin

Reactivity

Human, Rat, Mouse

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Actin antibody is un-conjugated

Application

Immunohistochemistry (IHC), ELISA

Discover our top product Actin Primary Antibody

Binding Specificity
16

15

14

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11

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 21-70, pTyr55

Specificity

Actin-pan (alpha/gamma) (Phospho-Tyr55/53) Antibody detects endogenous levels of Actin-pan only when phosphorylated at Tyr55/53.
PhosphorylationH:Y55/Y53 M:Y55/Y53 R:Y55/Y53

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity

> 95 %

Immunogen

The antiserum was produced against synthesized peptide derived from human Actin-pan around the phosphorylation site of Tyr55/53.

Isotype

IgG
anti-Actin antibody
Reactivity: Plant WB Host: Mouse Monoclonal Q30 unconjugated

anti-Actin (AA 165-260) antibody
Reactivity: Human, Rat, Mouse, Rabbit, Dog WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Actin (AA 300-380) antibody
Reactivity: Human, Rat, Mouse WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Actin antibody
Reactivity: Human, Rat, Mouse, Chicken, Dog, Cow, Pig, Guinea Pig WB, ELISA, FACS, IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-Actin (C-Term) antibody
Reactivity: Human, Rat, Mouse WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Actin antibody
Reactivity: Chicken WB, IHC, ELISA, ICC Host: Mouse Monoclonal C4 unconjugated

anti-Actin antibody
Reactivity: Arabidopsis, Oryza sativa, Nicotiana tabacum, Barley WB Host: Rabbit Polyclonal unconjugated

anti-Actin (AA 367-377) antibody
Reactivity: Human, Rat WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Actin (AA 21-70) antibody
Reactivity: Human, Rat, Mouse WB, IHC, IF, ELISA Host: Rabbit Polyclonal unconjugated

anti-Actin (AA 189-218) antibody
Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

Application Notes

IHC: 1:50~1:100 ELISA: 1:5000

Comment

Unigene-Number: Hs.709351, Hs.514581, Hs.516105, Hs.1288 (NCBI Gene Symbol: ACTC1)

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Stable at -20°C for at least 1 year.

Expiry Date

12 months
Target

Actin

Background

Synonyms: ACTA, ACTA1, ACTS, Actin, alpha skeletal muscle, Alpha-actin 1, ACTB, Actin, cytoplasmic 1, Beta-actin, ACTG, ACTG1, actin, cytoplasmic 2, gamma-actin
NCBI Gene Symbol: ACTC1

Molecular Weight

41 kDa

Gene ID

70, 71, 72

OMIM

102540

UniProt

P68032, P63261, P63267, P68133