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Actin antibody (pTyr55)

This Rabbit Polyclonal antibody specifically detects Actin in IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.
Catalog No. ABIN1531480

Quick Overview for Actin antibody (pTyr55) (ABIN1531480)

Target

See all Actin Antibodies
Actin

Reactivity

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Human, Rat, Mouse

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Actin antibody is un-conjugated

Application

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Immunohistochemistry (IHC), ELISA
  • Binding Specificity

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    AA 21-70, pTyr55

    Specificity

    Actin-pan (alpha/gamma) (Phospho-Tyr55/53) Antibody detects endogenous levels of Actin-pan only when phosphorylated at Tyr55/53.

    PhosphorylationH:Y55/Y53 M:Y55/Y53 R:Y55/Y53

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human Actin-pan around the phosphorylation site of Tyr55/53.

    Isotype

    IgG
  • Application Notes

    IHC: 1:50~1:100 ELISA: 1:5000

    Comment

    Unigene-Number: Hs.709351, Hs.514581, Hs.516105, Hs.1288 (NCBI Gene Symbol: ACTC1)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    Actin

    Background

    Synonyms: ACTA, ACTA1, ACTS, Actin, alpha skeletal muscle, Alpha-actin 1, ACTB, Actin, cytoplasmic 1, Beta-actin, ACTG, ACTG1, actin, cytoplasmic 2, gamma-actin

    NCBI Gene Symbol: ACTC1

    Molecular Weight

    41 kDa

    Gene ID

    70, 71, 72

    OMIM

    102540

    UniProt

    P68032, P63261, P63267, P68133
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