ADAM17 antibody (pThr735)

Catalog No. ABIN1531481

Product Details anti-ADAM17 Antibody

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Binding Specificity: AA 701-750, pThr735

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity:

ADAM 17 (Phospho-Thr735) Antibody detects endogenous levels of ADAM 17 only when phosphorylated at Thr735.

PhosphorylationH:T735 M:T735 R:T735

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ADAM 17 around the phosphorylation site of Thr735.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:20000

Comment:

Unigene-Number: Hs.404914 (NCBI Gene Symbol: ADAM17)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-ADAM17 antibody (ABIN1531481)

Bilousova, Taylor, Emirzian, Gylys, Frautschy, Cole, Teng: "Parallel age-associated changes in brain and plasma neuronal pentraxin receptor levels in a transgenic APP/PS1 rat model of Alzheimer's disease." in: Neurobiology of disease, Vol. 74, pp. 32-40, (2015) (PubMed).

Target Details for ADAM17

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: ADAM 17 (ADAM17 Products)

Background:

Synonyms: ADA17, ADAM 17, CD156b antigen, CSVP, TACE, TNF-alpha convertase, TNF-alpha-converting enzyme, a disintegrin and metalloproteinase domain 17, snake venom-like protease

NCBI Gene Symbol: ADAM17

Molecular Weight: 93 kDa

Gene ID: 6868

OMIM: 603639

UniProt: P78536

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus