ARAF antibody (pTyr302)

Catalog No. ABIN1531487

Product Details anti-ARAF Antibody

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Binding Specificity: AA 276-325, pTyr302

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARAF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity:

A-RAF (Phospho-Tyr302) Antibody detects endogenous levels of A-RAF only when phosphorylated at Tyr302.

PhosphorylationH:Y302 M:Y300 R:Y300

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

Comment:

Unigene-Number: Hs.225936, Hs.446641 (NCBI Gene Symbol: ARAF)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: A-RAF (ARAF Products)

Background:

Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, Proto-oncogene Pks, ARAF, ARAF1, PKS, PKS2

NCBI Gene Symbol: ARAF

Molecular Weight: 67 kDa

Gene ID: 369

OMIM: 311010

UniProt: P10398

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C