For best experience we recommend to activate Javascript in your browser.

ARAF antibody

Name: ARAF antibody
SKU: ABIN5557283
Availability: InStock

The Rabbit Monoclonal anti-ARAF antibody (Clone 24D1) (ABIN5557283) specifically detects ARAF in WB, IF (cc) and IHC (p). The antibody is reactive with Human and Mouse samples.

HeLa cell lysates probed with A-RAF (24D1) Monoclonal Antibody at 1:1000 overnight at 4˚C. (ARAF antibody)

Paraformaldehyde-fixed, paraffin embedded human kidney section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer at 37°C for 20min, Antibody incubation with A-RAF (24D1) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining. (ARAF antibody)

IHC (p)

NIH/3T3 cells were stained with A-RAF (24D1) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection. (ARAF antibody)

IF (cc)

HeLa cells were stained with A-RAF (24D1) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection. (ARAF antibody)

IF (cc)

4 images

Catalog No. ABIN5557283

$490.77

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 8 to 12 Business Days

Quick Overview for ARAF antibody (ABIN5557283)

Target

See all ARAF Antibodies

ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivity

Human, Mouse

Host

Rabbit

Clonality

Monoclonal

Conjugate

This ARAF antibody is un-conjugated

Application

Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

24D1

Discover our top product ARAF Primary Antibody

Cross-Reactivity

Human, Mouse

Purification

Purified by Protein A.

Immunogen

Recombinant human A-RAF protein, around 150-300aa.

Isotype

IgG
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 151-250) antibody
ARAF Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 3E2 unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-606) antibody
ARAF Reactivity: Human WB, IF, PLA Host: Rabbit Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (C-Term) antibody
ARAF Reactivity: Human, Mouse WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 160-310) antibody
ARAF Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) antibody
KD Validated ARAF Reactivity: Human WB, FACS Host: Rabbit Monoclonal 25GB440 unconjugated Recombinant Antibody

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Internal Region) antibody
ARAF Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pTyr301), (pTyr302) antibody
ARAF Reactivity: Human, Mouse WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pSer299) antibody
ARAF Reactivity: Human WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 256-286) antibody
ARAF Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB03325 unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 276-325), (pTyr302) antibody
ARAF Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name

A-RAF

Background

Synonyms: Serine/threonine-protein kinase A-Raf, ARAF, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene Pks, ARAF1, PKS, PKS2
Background: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.

Gene ID

369

UniProt

P10398

Pathways

MAPK Signaling, RTK Signaling, Hepatitis C