ARAF antibody (Internal Region)

Catalog No. ABIN6259664

This Rabbit Polyclonal antibody specifically detects ARAF in WB, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ARAF antibody (Internal Region) (ABIN6259664)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARAF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ARAF Antibody

Binding Specificity: Internal Region

Specificity: A-RAF Antibody detects endogenous levels of total A-RAF.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human A-RAF, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: ARAF

Background:

Description: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.

Gene: ARAF

Molecular Weight: 67 kDa

Gene ID: 369

UniProt: P10398

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C