BCL2L1 antibody (pThr47)

Catalog No. ABIN1531492

This Rabbit Polyclonal antibody specifically detects BCL2L1 in WB, IHC, IF and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for BCL2L1 antibody (pThr47) (ABIN1531492)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL2L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-BCL2L1 Antibody

Binding Specificity: AA 13-62, pThr47

Purpose: Bcl-x (phospho Thr47) Antibody

Specificity: Phospho-Bcl-x (T47) Polyclonal Antibody detects endogenous levels of Bcl-x protein only when phosphorylated at T47.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human BCL-XL around the phosphorylation site of Thr47. AA range:13-62

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Alternative Name: BCL-XL

Background:

Synonyms: BCL2L1, BCL2L, BCLX, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X

Background: The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015],

Gene Name: BCL2L1

Protein Name: Bcl-2-like protein 1

Molecular Weight: 26 kDa

Gene ID: 598

OMIM: 600039

UniProt: Q07817

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling