PTK6 antibody (pTyr447)

Catalog No. ABIN1531497

The Rabbit Polyclonal anti-PTK6 antibody (ABIN1531497) specifically detects PTK6 in WB, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PTK6 antibody (pTyr447) (ABIN1531497)

Target: PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTK6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-PTK6 Antibody

Binding Specificity: AA 402-451, pTyr447

Purpose: Brk (phospho Tyr447) Antibody

Specificity: Phospho-Brk (Y447) Polyclonal Antibody detects endogenous levels of Brk protein only when phosphorylated at Y447.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Breast Tumor Kinase around the phosphorylation site of Tyr447. AA range:402-451

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PTK6

Target: PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))

Alternative Name: Breast Tumor Kinase

Background:

Synonyms: PTK6, BRK, Protein-tyrosine kinase 6, Breast tumor kinase, Tyrosine-protein kinase BRK

Background: protein tyrosine kinase 6(PTK6) Homo sapiens The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012],

Gene Name: PTK6

Protein Name: Protein-tyrosine kinase 6

Molecular Weight: 51 kDa

Gene ID: 5753

OMIM: 602004

UniProt: Q13882