PKC epsilon antibody (pSer729)

Catalog No. ABIN1531507

This Rabbit Polyclonal antibody specifically detects PKC epsilon in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PKC epsilon antibody (pSer729) (ABIN1531507)

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC epsilon antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PKC epsilon Antibody

Binding Specificity: AA 688-737, pSer729

Specificity:

PKC epsilon (Phospho-Ser729) Antibody detects endogenous levels of PKC epsilon only when phosphorylated at Ser729.

PhosphorylationH:S729 M:S729 R:S729

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC epsilon around the phosphorylation site of Ser729.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:10000

Comment:

Unigene-Number: Hs.580351 (NCBI Gene Symbol: PRKCE)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PKC epsilon

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Alternative Name: PKC epsilon

Background:

Synonyms: KPCE, PKC-epsilon, PKCEA, PRKCE, Protein kinase C, epsilon type, kinase PKC-epsilon, nPKC-epsilon

NCBI Gene Symbol: PRKCE

Molecular Weight: 83 kDa

Gene ID: 5581

OMIM: 176975

UniProt: Q02156

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling