CAMK1 antibody (pThr177)

Catalog No. ABIN1531530

The Rabbit Polyclonal anti-CAMK1 antibody (ABIN1531530) specifically detects CAMK1 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CAMK1 antibody (pThr177) (ABIN1531530)

Target: CAMK1 (Calcium/calmodulin-Dependent Protein Kinase I (CAMK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CAMK1 Antibody

Binding Specificity: AA 143-192, pThr177

Purpose: CaMKIα (phospho Thr177) Antibody

Specificity: Phospho-CaMKIα (T177) Polyclonal Antibody detects endogenous levels of CaMKIα protein only when phosphorylated at T177.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CaMK1-alpha around the phosphorylation site of Thr177. AA range:143-192

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for CAMK1

Target: CAMK1 (Calcium/calmodulin-Dependent Protein Kinase I (CAMK1))

Background:

Synonyms: CAMK1, Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM-KI, CaM kinase I alpha, CaMKI-alpha

Background: Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008],

Gene Name: CAMK1

Protein Name: Calcium/calmodulin-dependent protein kinase type 1

Molecular Weight: 41 kDa

Gene ID: 8536

OMIM: 604998

UniProt: Q14012

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Smooth Muscle Cell Migration