Caspase 3 antibody (pSer150)

Catalog No. ABIN1531535

The Rabbit Polyclonal anti-Caspase 3 antibody has been validated for WB, IHC and ELISA. It is suitable to detect Caspase 3 in samples from Human, Mouse and Rat.

Quick Overview for Caspase 3 antibody (pSer150) (ABIN1531535)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 116-165, pSer150

Specificity:

Caspase 3 (Phospho-Ser150) Antibody detects endogenous levels of Caspase 3 only when phosphorylated at Ser150.

PhosphorylationH:S150 M:S150 R:S150

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 3 around the phosphorylation site of Ser150.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

Comment:

Unigene-Number: Hs.141125 (NCBI Gene Symbol: CASP3)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase 3

Background:

Synonyms: Apopain precursor, CASP-3, CPP-32, CPP32, Caspase-3, Cysteine protease CPP32, ICE3, SCA-1, SREBP cleavage activity 1, Yama protein

NCBI Gene Symbol: CASP3

Molecular Weight: 31 kDa

Gene ID: 836

OMIM: 600636

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation