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CD5 antibody (pTyr453)

Name: CD5 antibody (pTyr453)
SKU: ABIN1531547
Price: 341 USD
Availability: InStock

CD5 Reactivity: Human, Mouse, Rat WB, IF, ELISA Host: Rabbit Polyclonal unconjugated

Western blot analysis of extracts from 293 cells treated with PMA 125ng/ml 30' , using CD5 (Phospho-Tyr453) Antibody. (CD5 antibody (pTyr453))

Immunofluorescence analysis of HepG2 cells, using CD5 (Phospho-Tyr453) Antibody. (CD5 antibody (pTyr453))

2 images

Catalog No. ABIN1531547

Datasheet as PDF

Target See all CD5 Antibodies

CD5
Binding Specificity
16

9

8

6

5

5

5

5

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 421-470, pTyr453
Reactivity
220

89

57

27

22

16

15

15

13

12

11

10

5

2

1

1

1
Human, Mouse, Rat
Host
167

79

56

1
Rabbit
Compatible Secondaries
Clonality
232

69
Polyclonal
Conjugate
108

38

32

28

13

8

6

5

4

4

4

4

4

4

4

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD5 antibody is un-conjugated
Application
236

107

67

65

65

52

30

29

22

18

13

13

12

6

5

3

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), ELISA

Specificity

CD5 (Phospho-Tyr453) Antibody detects endogenous levels of CD5 only when phosphorylated at Tyr453.
PhosphorylationH:Y453 M:Y452 R:Y449

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity

> 95 %

Immunogen

The antiserum was produced against synthesized peptide derived from human CD5 around the phosphorylation site of Tyr453.

Isotype

IgG

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Application Notes

WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

Comment

Unigene-Number: Hs.58685 (NCBI Gene Symbol: CD5)

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Stable at -20°C for at least 1 year.

Expiry Date

12 months
Target

CD5

Alternative Name

CD5 (CD5 Products)

Synonyms

CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody

Background

Synonyms: LEU1, Lymphocyte antigen CD5, Lymphocyte glycoprotein Ly-1, Lymphocyte glycoprotein T1/Leu-1, Lyt-1, T-cell surface glycoprotein CD5 precursor
NCBI Gene Symbol: CD5

Molecular Weight

54 kDa

Gene ID

921

OMIM

153340

UniProt

P06127