DDIT3 antibody (pSer30)

Catalog No. ABIN482168

The Rabbit Polyclonal anti-DDIT3 antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect DDIT3 in samples from Human and Mouse.

Quick Overview for DDIT3 antibody (pSer30) (ABIN482168)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-DDIT3 Antibody

Binding Specificity: AA 15-64, pSer30

Specificity:

CHOP (Phospho-Ser30) Antibody detects endogenous levels of CHOP only when phosphorylated at Ser30.

PhosphorylationH:S30 M:S30

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CHOP around the phosphorylation site of Ser30.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

Comment:

Unigene-Number: Hs.505777, Hs.690217 (NCBI Gene Symbol: DDIT3)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Alternative Name: CHOP

Background:

Synonyms: C/EBP-homologous protein, DDIT3, DNA-damage inducible transcript 3, GADD153, Growth arrest and DNA-damage-inducible protein GADD153

NCBI Gene Symbol: DDIT3

Molecular Weight: 19 kDa

Gene ID: 1649

OMIM: 126337

UniProt: P35638

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis