DDX5 antibody (pTyr593)

Catalog No. ABIN1531579

This Rabbit Polyclonal antibody specifically detects DDX5 in WB, IHC, IF and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DDX5 antibody (pTyr593) (ABIN1531579)

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-DDX5 Antibody

Binding Specificity: AA 565-614, pTyr593

Purpose: p68 RNA Helicase (phospho Tyr593) Antibody

Specificity: Phospho-p68 RNA Helicase (Y593) Polyclonal Antibody detects endogenous levels of p68 RNA Helicase protein only when phosphorylated at Y593.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human DDX5/DEAD-box Protein 5 around the phosphorylation site of Tyr593. AA range:565-614

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000.IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for DDX5

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Alternative Name: DDX5/DEAD-Box Protein 5

Background:

Synonyms: DDX5, G17P1, HELR, HLR1, Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],

Gene Name: DDX5

Protein Name: Probable ATP-dependent RNA helicase DDX5

Molecular Weight: 69 kDa

Gene ID: 1655

OMIM: 180630

UniProt: P17844

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus