HDAC3 antibody (pSer424)

Catalog No. ABIN1531607

The Rabbit Polyclonal anti-HDAC3 antibody is suitable to detect HDAC3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for HDAC3 antibody (pSer424) (ABIN1531607)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-HDAC3 Antibody

Binding Specificity: AA 379-428, pSer424

Purpose: HDAC3 (phospho Ser424) Antibody

Specificity: Phospho-HDAC3 (S424) Polyclonal Antibody detects endogenous levels of HDAC3 protein only when phosphorylated at S424.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HDAC3 around the phosphorylation site of Ser424. AA range:379-428

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for HDAC3

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Alternative Name: HDAC3

Background:

Synonyms: HDAC3, Histone deacetylase 3, HD3, RPD3-2, SMAP45

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA.The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008],

Gene Name: HDAC3

Protein Name: Histone deacetylase 3

Molecular Weight: 48 kDa

Gene ID: 8841

OMIM: 605166

UniProt: O15379

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development