ERBB3 antibody (pTyr1222)

Catalog No. ABIN1531609

This Rabbit Polyclonal antibody specifically detects ERBB3 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for ERBB3 antibody (pTyr1222) (ABIN1531609)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-ERBB3 Antibody

Binding Specificity: AA 1191-1240, pTyr1222

Specificity:

HER3 (Phospho-Tyr1222) Antibody detects endogenous levels of HER3 only when phosphorylated at Tyr1222.

PhosphorylationH:Y1222 H:Y1219 H:Y1219

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HER3 around the phosphorylation site of Tyr1222.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

Comment:

Unigene-Number: Hs.118681 (NCBI Gene Symbol: ERBB3)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: HER3

Background:

Synonyms: c-erbB3, ERB3, ERBB3, HER3, kinase ErbB3, Receptor protein-tyrosine kinase erbB-3 precursor, Tyrosine kinase-type cell surface receptor HER3

NCBI Gene Symbol: ERBB3

Molecular Weight: 148 kDa

Gene ID: 2065

OMIM: 190151

UniProt: P21860

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway