Vinculin antibody (pTyr821)

Catalog No. ABIN1531640

The Rabbit Polyclonal anti-Vinculin antibody has been validated for IHC, ELISA and IF. It is suitable to detect Vinculin in samples from Human, Mouse and Rat.

Quick Overview for Vinculin antibody (pTyr821) (ABIN1531640)

Target: Vinculin (VCL)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Vinculin Antibody

Binding Specificity: AA 786-835, pTyr821

Specificity:

Vinculin (Phospho-Tyr821) Antibody detects endogenous levels of Vinculin only when phosphorylated at Tyr821.

PhosphorylationH:Y821 M:Y821 R:Y822

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Vinculin around the phosphorylation site of Tyr821.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

Comment:

Unigene-Number: Hs.643896 (NCBI Gene Symbol: VINC)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background:

Synonyms: Metavinculin, VCL, VINC

NCBI Gene Symbol: VINC

Molecular Weight: 123 kDa

Gene ID: 7414

OMIM: 193065

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2